The hydrophobic nature of the pig intrinsic factor receptor in the intestine |
| |
Authors: | Ilkka Kouvonen |
| |
Institution: | Minerva Institute for Medical Research, P.O. Box 819, SF-00101 Helsinki 10 Finland |
| |
Abstract: | The pig intestinal intrinsic factor receptor has been isolated and dissociated into its α and β subunits. The β subunit was found to be more hydrophobic than the α subunit. In a detergent solution only the α subunit was accessible to digestion with papain. The whole isolated receptor was introduced into artificial single bilayer liposomes where is apparently was randomly oriented. Liposomes containing the receptor were digested with papain and the polypeptide segments that stayed in the lipid fraction were extracted and analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Four species were found with values of 23 000, 45 000, 70 000 and 86 000. |
| |
Keywords: | Cobalamin Intrinsic factor receptor Papain digestion Subunit structure Vitamin B-12 (Pig gastric mucosa) SDS sodium dodecyl sulfate |
本文献已被 ScienceDirect 等数据库收录! |