Tyrosine transport by membrane vesicles isolated from rat brain |
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Authors: | M.Carmen Aragón Cecilio Giménez Federico Mayor Juan G. Marvizón Fernando Valdivieso |
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Affiliation: | Departamento de Bioquimica y Biologia Molecular, Centro de Biologia Molecular, Facultad de Ciencias, Universidad Autónoma, Madrid-34 Spain |
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Abstract: | Tyrosine uptake by membrane vesicles derived from rat brain has been investigated. The uptake is dependent on an Na+ gradient ([). The uptake is transport into an osmotically active space and not a binding artifact as indicated by the effect of increasing the medium osmolarity. The process is stimulated by a membrane potential (negative inside) as demonstrated by the effect of the ionophores valinomycin and carbonyl cyanide and anions with different permeabilities. Kinetic data show that tyrosine is accumulated by two systems with different affinities. Tyrosine uptake is inhibited by the presence of phenylalanine and tryptophan. |
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Keywords: | Tyrosine transport Membrane vesicle (Rat brain) CCCP triphenylmethylphosphonium ion |
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