Electrophoretic analysis of the plasma membrane proteins of a mutant of Neurospora crassa which lacks a cell wall |
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Authors: | Ronald L. Stotish Ethel W. Somberg |
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Affiliation: | Biochemistry Laboratory, Department of Zoology and Physiology, Rutgers, The State University, Newark, NJ 07102 U.S.A. |
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Abstract: | The plasma membrane proteins of a mutant of Neurospora crassa (FGSC No. 326) which lacks a cell wall were analyzed by two-dimensional polyacrylamide gel electrophoresis. Approximately 180 different proteins were detected in purified plasma membrane preparations. Nonpermeant labeling experiments indicated that approximately 40% of these proteins were exposed on the extracytoplasmic surface of the plasma membranes of these cells. The studies demostrate the complexity of the protein composition of N. crassa 326 plasma membranes to be greater than has been suggested by previous investigations. |
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Keywords: | Plasma membrane protein Surface labeling two-dimensional Polyacrylamide gel electrophoresis (Neurospora crassa) SDS sodium dodecyl sulfate |
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