The diffusion-concentration product of oxygen in lipid bilayers using the spin-label <span class="math"><math><mtext>T</mtext><msub><mi></mi><mn>1</mn></msub></math></span> method期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

The diffusion-concentration product of oxygen in lipid bilayers using the spin-label $T_{1}$ method

Authors:	W.K. Subczynski James S. Hyde

Affiliation:	National Biomedical ESR Center, Department of Radiology, The Medical College of Wisconsin, Milwaukee, WI 53226 U.S.A.

Abstract:	A method is described to measure the oxygen diffusion-concentration product, $D_{o} [O_{2}]$ , at any locus that can be probed or labeled using nitroxide radicals. The method is based on the dependence of the spin-lattice relaxation time $T_{1}$ of the spin label on the bimolecular collision rate with oxygen. Strong Heisenberg exchange between spin label and oxygen contributes directly to $T_{1}$ of the spin label, while dipolar interactions are negligible. Both time-domain and continuous wave saturation methods for studying $T_{1}$ are considered. The method has been applied to phospholipid liposomes using fatty acid spin labels. A discontinuity in $D_{o} [O_{2}]$ at the main phase transition was observed.

Keywords:	Oxygen diffusion Oxygen concentration Heisenberg exchange Spin label Spin lattice relaxation ESR (Lipid bilayer) DMPC L-α-dimyristoylphosphotidylcholine DPPC L-α-dipalmitoylphosphatidylcholine ELDOR electron-electron double resonance Hepes To whom correspondence should be addressed. P.O. Box 26509 should be added to the address given.
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