EPR characteristics of oxygen-evoloving Photosytem II particles from Phormidium laminosum |
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| Authors: | JHA Nugent AC Stewart MCW Evans |
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| Institution: | 1. Department of Botany and Microbiology, University College London, London WC1E 6BT U.K.;2. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW U.K. |
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| Abstract: | The EPR characteristics of oxygen evolving particles prepared from Phormidium laminosum are described. These particles are enriched in Photosystem II allowing EPR investigation of signals which were previously small or masked by those from Photosystem I in other preparations. EPR signals from a Signal II species and high potential cytochrome b-559 appear as they are photooxidised at cryogenic temperatures by Photosystem II. The Signal II species is a donor close to the Photosystem II reaction centre and may represent part of the charge accumulation system of water oxidation. An EPR signal from an iron-sulphur centre which may represent an unidentified component of photosynthetic electron transport is also described.The properties of the oxygen evolving particles show that the preparation is superior to chloroplasts or unfractionated algal membranes for the study of Photosystem II with a functional water oxidation system. |
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| Keywords: | ESR Photosystem II Oxygen evolution (Blue-green alga Phormidium laminosum) DCMU 3-(3′ 4′-dichlorophenyl)-1 1-dimethylurea |
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