Energization of sulfate transport in yeast |
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Authors: | J Horák L ?íhová A Kotyk |
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Institution: | Department of Cell Physiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Videňská, 1083, 142 20 Prague 4 Czechoslovakia |
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Abstract: | Sulfate uptake by Saccharomyces cerevisiae is stimulated about 12-fold by preincubation of cells with 1% d-glucose or 1% ethanol. The remains unchanged (0.34–0.38 mM), the increase from 18–20 to 195–230 and 170–185 nmol/min per g dry wt., respectively, after glucose and ethanol preincubation. The stimulation involves protein synthesis (it is suppressed by cycloheximide), has a half-time of 18 min and requires mitochondrial respiration (no or low effect in respiration-deficient mutants and those lacking ADP-ATP transport in mitochondria, as well as after anaerobic preincubation of the wild-type strain, and in low-phosphate cells). The presence of NH4+ and some amino acids (e.g., leucine, aspartate, cysteine and methionine) depressed the stimulation while that of cationic amino acids (typically arginine and lysine) and of K+ increased it by 50–80%. The stimulated (i.e., newly synthesized) transport system was degraded with a half-life of about 10 min. |
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Keywords: | Sulfate transport Transport stimulation (Saccharomyces cerevisiae) |
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