The subunit structure of bovine heart mitochondrial transhydrogenase |
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Authors: | W.Marshall Anderson Ronald R. Fisher |
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Affiliation: | Department of Chemistry, University of South Carolina, Columbia, SC 29208 U.S.A. |
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Abstract: | Reaction of purified bovine heart transhydrogenase with bifunctional cross-linking reagents dimethyl adipimidate, dimethyl pimelimidate, dimethyl suberimidate, and dithiobis(succinimidyl propionate) results in the appearance of a dimer band on sodium dodecyl sulfate polyacrylamide gels with no higher oligomers formed. Treatment of the enzyme with 6 M urea led to inactivation and prevented cross-linking by dimethyl suberimidate. Transhydrogenase reconstituted into phosphatidylcholine proteoliposomes also yielded a dimer band on cross-linking. These data indicate that soluble and functionally reconstituted transhydrogenase possesses a dimeric structure. |
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Keywords: | Transhydrogenase subunit Cross-linking (Bovine heart mitochondria) |
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