Acidification and sodium entry in frog skin epithelium |
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Authors: | Dale J. Benos |
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Affiliation: | Department of Physiology and Laboratory of Human Reproduction and Reproductive Biology, Harvard Medical School, 45 Shattuck Street, Boston, MA 02115 U.S.A. |
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Abstract: | Acidification of the external medium by isolated frog skin epithelium (Rana catesbeiana, Rana temporaria, and Caudiververa caudiververa) and its relationship to Na+ uptake was studied. Acidification was measured by the pH-stat technique under short-circuit or open-circuit conditions. The results of this study demonstrate that (a) acidification by these species of in vitro frog skins is not directly coupled to Na+ or anion transport; (b) acidification can be inhibited by the diuretic drug amiloride, but only at high external Na+ concentrations; (c) acidification rate in these species of frog skin is controlled in part by the metabolic production of CO2; and (d) the positive correlation between net Na+ absorption and net acidification observed in whole animal studies could not be replicated in the in vitro skin preparation, even when the frogs were first chronically stressed by salt depletion, a physiological state comparable to that used in the in vivo experiments. |
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Keywords: | Acidification Amiloride inhibition (Frog skin epithelium) ADH antidiuretic hormone DIDS 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid Hepes |
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