Electrochemical titrations of a ferredoxin-ferredoxin:NADP+ oxidoreductase complex |
| |
| Authors: | JMichael Smith Wayne H Smith David B Knaff |
| |
| Institution: | Department of Chemistry, Texas Tech University, Lubbock, TX 79409 U.S.A. |
| |
| Abstract: | Potentiometric titrations employing an electrochemical thin-layer cell indicate that complex formation between ferredoxin and ferredoxin:NADP+ oxidoreductase alters the midpoint oxidation-reduction potentials of both proteins. The midpoint potential of ferredoxin in the complex becomes 22 ± 6 mV more negative compared to ferredoxin alone while the midpoint potential of ferredoxin:NADP+ oxidoreductase becomes 23 ± 4 mV more positive on complex formation. |
| |
| Keywords: | Electrochemical titration Ferredoxin oxidation-reduction midpoint potential CD circular dichroism |
| 本文献已被 ScienceDirect 等数据库收录！ |
