Hexane-solubilised reaction centre proteolipid complexes of Rhodopseudomonas sphaeroides

Reaction centres from Rhodopseudomonas sphaeroides, strain R-26, have been solubilised in hexane by the use of phospholipids and cations. Two procedures have been developed: (I) a two-step technique involving the formation of detergent-free proteoliposomes from detergent solubilised reaction centres and phospholipids and mixing these with hexane in the presence of cations; (II) directly sonicating detergent-solubilised reaction centres with phosopholipids before mixing with hexane and cations.The yield of the extracted complex varied with the ratios of protein, phospholipid and cations, species of phospholipid, and sonication time. The spectral characteristics of the complex in the organic phase were similar to those of detergent-solubilised reaction centres. The stability of the reaction centres appeared to be dependent on the presence of phospholipid and water in the hexane. The usefulness of the hexane solution as a model membrane system is discussed and its possible future applications are considered.