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Replication of the single-stranded DNA bacteriophage M13: absence of intracellular phages
Authors:U Stegen  P H Hofschneider
Affiliation:1. Department of Life Sciences, Imperial College London, London, United Kingdom;2. Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, United Kingdom;2. Howard Hughes Medical Institute, The Rockefeller University, New York, NY, United States
Abstract:In Escherichia coli cultures infected with the filamentous single-stranded DNA bacteriophage M13, mature phages cannot be detected intracellularly at any time after infection, even though as many as 1000 extracellular phages may be found per infected cell. Even at very late stages after infection, no more than about 300 intracellular infectious phage DNA molecules and no more than about 110 phage coat equivalents per infected cell can be detected.
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