Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone

Experiments were performed under several conditions seeking evidence for turnover of protein-bound lipoic acid in Escherichia coli analogous to that described for the 4′-phosphopantetheine moiety of the E. coli acyl carrier protein. Pulse-chase, chase experiments using both low and saturating concentrations of lipoic acid, chase experiments in the presence of chloramphenicol, which prevents incorporation of lipoic acid into the protein-bound form, and chase experiments in cells in which the free pool of lipoic acid was reduced by osmotic shock all failed to demonstrate any turnover of protein-bound lipoic acid.