Alterations of gene expression in potato (Solanum commersonii) during cold acclimation |
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| Authors: | M. J. Tseng P. H. Li |
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| Affiliation: | Lab. of Plant Hardiness, Dept of Horticultural Science, Univ. of Minnesota, St. Paul, MN 554108;Agricultural Biotechnology Laboratories, National Chung-Hsing University, Taichung, Taiwan |
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| Abstract: | Plantlets of Solanum commersonii stem-culture were acclimated at 5°C day/night temperature for 14 days. Cold hardiness increased from – 3.5°C to – 8.6°C. During the course of acclimation, the synthesis of polypeptides was investigated and poly (A+) RNA was isolated. Translation products of poly(A+) RNA in a rabbit rcticulocyte lysate system were then analyzed. During the 14 days of acclimation, 23 cold-induced polypeptides were identified. Most of them disappeared following 1 day of de-acclimation at a 20/15°C day/night regime. The synthesis of one group of polypeptides is prominent and stable throughout the acclimation period. The other group is transient. The most prominent and stable polypeptides have molecular weights of 21, 22, 31 and 83 kDa.
Acclimation alters translatable mRNA population during the development of cold hardiness. Two mRNAs encoding in vitro translation products at 26 and 27 kDa were identified during the course of acclimation. These proteins may play important roles in the overall programming for the development of cold hardiness in tuber-bearing S. commersonii. |
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| Keywords: | Cold hardiness poly(A+) RNA polypeptides potato protein synthesis Solanium commersonii. |
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