Production of laccase byCurvularia sp. |
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Authors: | U C Banerjee R M Vohra |
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Institution: | (1) Institute of Microbial Technology, 160 014 Chandigarh, India |
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Abstract: | ACurvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular
laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30°C
were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found
to be 5.2 and 50°C, respectively. Under optimum conditions, the enzyme had aK
m (guaiacol) of 0.75 mmol/L and aV of 1.50 CU min−1 ml−1. Some divalent metal ions inhibited laccase activity at very low concentrations. |
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