Characterization of embryogenic mango cultures selected for resistance to Colletotrichum gloeosporioides culture filtrate and phytotoxin |
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Authors: | S Jayasankar R E Litz |
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Institution: | (1) Tropical Research and Education Center, University of Florida, 18905 SW 280 St, Homestead, FL 33031-3314, USA, US |
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Abstract: | Embryogenic nucellar cultures of two polyembryonic mango cultivars, ‘Hindi’ and ‘Carabao’, were selected for resistance to
the culture filtrate and phytotoxin of a virulent strain of Colletotrichum gloeosporioides Penz. that was isolated from mango leaves. The cultures were recurrently selected either with progressively increasing concentrations
of culture filtrate or by continuous challenge with the same concentration of either culture filtrate phytotoxin. Mycelium
growth was inhibited when the pathogen was cocultured with the selected, resistant embryogenic cultures. Conditioned plant
growth medium containing macerated resistant embryogenic cultures did not inhibit mycelium growth, confirming that extracellular
antifungal compounds were involved in the defense response. Enhanced secretion of chitinase and glucanase was observed in
the plant growth medium in which resistant embryogenic cultures and regenerated somatic embryos were grown in comparison with
the controls.
Received: 6 February 1997 / Accepted: 4 November 1997 |
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Keywords: | Mangifera indica L Anthracnose Somatic embryogenesis Dual culture RAPD |
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