Enzyme levels in cultured astrocytes,oligodendrocytes and Schwann cells,and neurons from the cerebral cortex and superior cervical ganglia of the rat |
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Authors: | Robert S Rust Jr Joyce G Carter David Martin Jeanne M Nerbonne Patricia A Lampe Mary Ellen Pusateri Oliver H Lowry |
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Institution: | (1) Departments of Molecular Biology and Pharmacology and Neurology and Pediatrics, Washington University School of Medicine, 63110 St. Louis, MO;(2) The Center for Studies of Higher Brain Function, Washington University School of Medicine, 63110 St. Louis, MO;(3) Departments of Neurology and Pediatrics, University of Wisconsin, School of Medicine, 53792 Madison, WI |
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Abstract: | Data are presented for 16 enzymes from 8 metabolic systems in cell cultures consisting of approximately 95% astrocytes and 5% oligodendrocytes. Nine of these enzymes were also measured in cultures of oligodendrocytes, Schwann cells, and neurons prepared from both cerebral cortex and superior cervical ganglia. Activities, in mature astrocyte cultures, expressed as percentage of their activity in brain, ranged from 9% for glycerol-3-phosphate dehydrogenase to over 300% for glucose-6-phosphate dehydrogenase. Creatine phosphokinase activity in astrocytes was about the same as in brain, half as high in oligodendrocytes, but 7% or less of the brain level in Schwann cells and superior cervical ganglion neurons and only 16% of brain in cortical neurons. Three enzymes which generate NADPH, the dehydrogenases for glucose-6-phosphate and 6-phosphogluconate, and the NADP-requiring isocitrate dehydrogenase, were present in astrocytes at levels at least twice that of brain. Oligodendrocytes had enzyme levels only 30% to 70% of those of astrocytes. Schwann cells had much higher lactate dehydrogenase and 6-phosphogluconate dehydrogenase activities than oligodendrocytes, but showed a remarkable similarity in enzyme pattern to those of cortical and superior cervical ganglion neurons.Special issue dedicated to Dr. Lewis Sokoloff. |
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Keywords: | Cultured cell enzymes astrocytes oligodendrocytes Schwann cells neurons |
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