Gene transfer into insect brain and cell-specific expression of bombyxin gene |
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Authors: | Ken’ichi Moto Salah Eldin Abdel Salam S Sakurai M Iwami |
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Institution: | (1) Department of Biology, Faculty of Science and Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan E-mail: masafumi@kenroku.kanazawa-u.ac.jp Fax: +81-76-2645977/2344140, JP |
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Abstract: | A transgene reporter consisting of the bombyxin gene promoter and the green fluorescent protein coding region was introduced
into intact brains of the silkworm Bombyx mori by in vitro electroporation. After in vitro culture of the brains, the fluorescence derived from the introduced reporter
gene was observed in all cases in eight neurosecretory cells that had previously been identified as bombyxin-producing cells
(BPCs). Although the fluorescence was not always observed in all cells, it was specific to BPCs, indicating that the reporter
was under the control of the bombyxin gene promoter in a BPC-specific manner. Electroporatical introduction of a reporter
gene was therefore found to be a suitable method for analyzing cell-specific expression in intact tissues and to be substitute
for germ-line transmission of reporters in the transgenic system. Application of this technique enables us to analyze the
cell-specific expression of transgene reporters within a few days and treat more than several dozens of the reporters within
1 month, which is difficult to do with the transgenic system.
Received: 8 December 1998 / Accepted: 8 March 1999 |
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Keywords: | Bombyxin Insulin Bombyx mori Electroporation Cell-specific expression |
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