Cloning and expression analyses of AtMRP4 , a novel MRP -like gene from Arabidopsis thaliana |
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| Authors: | R Sánchez-Fernández W Ardiles-Díaz M Van Montagu D Inzé and M J May |
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| Institution: | (1) Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute of Biotechnology, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Fax: +32-9-264-5349; e-mail: mamon@gengenp.rug.ac.be, BE;(2) Laboratoire Associé de l'Institut National de la Recherche Agronomique (France), Universiteit Gent, B-9000 Gent, Belgium, BE |
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| Abstract: | In all organisms glutathione-conjugate transporters (GS-X pumps) mediate the detoxification of a number of xenobiotics by
removing them from the cytosol. In addition, GS-X pumps appear to play a role in the processing of endogenous compounds. We
have isolated a novel genomic clone from Arabidopsis thaliana that encodes a putative GS-X pump, AtMRP4, which is part of a recently defined gene family. The derived amino acid sequence shares high levels of similarity (55–63%)
with human, yeast, and other Arabidopsis homologues. The expression of the different members of the AtMRP gene family in Arabidopsis cell suspensions after treatment with chemicals that modify glutathione metabolism (compounds that induce different types
of stress and that act as herbicide antidotes – safeners – in monocotyledonous species) revealed that the members of this
gene family are differentially regulated.
Received: 20 February 1998 / Accepted: 9 March 1998 |
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| Keywords: | Arabidopsis thaliana Glutathione-conjugate pump Stress Xenobiotic detoxification |
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