Increased level of calcitonin mRNA after 1,25-dihydroxyvitamin D3 injection in the rat |
| |
Authors: | N Segond B Legendre E H Tahri P Besnard A Jullienne M S Moukhtar J M Garel |
| |
Institution: | 1. Unité 113 de l''INSERM, CHU Saint-Antoine, 27 rue de Chaligny, 75571 Paris Cédex 12 France;2. Groupe Hormones du Calcium et Développement, Université Pierre et Marie Curie, 4 place Jussieu, 75230 Paris Cédex 05, France |
| |
Abstract: | Vitamin D metabolites are able to change plasma calcitonin (CT) levels, but nothing is known about a possible effect at the CT gene level. Here we have investigated the acute effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on the CT biosynthetic activity of thyroid glands from adult rats. Plasma CT levels were significantly increased (X2) 1 and 2 h after 1,25-(OH)2D3 injection in the face of unchanged plasma calcium values. The thyroidal CT content also was unchanged. A 2-fold increase in CT mRNA level measured by dot-blot hybridization occurred 1 and 2 h after 1,25-(OH)2D3 administration. Expression of CT gene products was examined in the rabbit reticulocyte lysate cell-free translation assay. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographed and quantified by integration. A single precursor of Mr approximately equal to 15 000 could be specifically immunoprecipitated with CT antisera. A 3-4-fold rise in translatable CT mRNA activity was observed 1 and 2 h after 1,25-(OH)2D3 injection. Thus, parallel changes in CT mRNA level, CT mRNA activity and plasma CT levels were observed in adult female rats after administration of 1,25-(OH)2D3. These findings demonstrate for the first time that 1,25-(OH)2D3 enhanced CT gene expression in the face of unchanged plasma calcium levels. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|