Removal of browning and growth enhancement by polyvinylpolypyrrolidone in protoplast cultures ofCyamopsis tetragonoloba L. |
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| Authors: | Praveen K Saxena R Gill |
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| Institution: | 1. Department of Botany, University of Delhi, 1007, Delhi
3. Bio-Organic Division, Bhabha Atomic Research Centre, Bombay, India
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| Abstract: | The occurrence of browning in protoplast cultures ofCyamopsis tetragonoloba completely inhibited the growth of protoplast derived colonies. Of the various additives employed to counteract the problem of browning and subsequent necrosis, polyvinylpolypyrrolidone (PVPP) was found most effective. Simultaneous addition of polyvinylpyrrolidone (PVP) to the protoplast culture medium accentuated the effect of PVPP and also improved the frequency of protoplast division. |
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