Nucleotide distribution in bacterial DNA's differing in G + C content |
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Authors: | Hideo Yamagishi |
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Institution: | (1) Molecular Biology Laboratory, Department of Biophysics, Faculty of Science, Kyoto University, Kyoto, Japan |
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Abstract: | Summary The DNA's ofMicrococcus lysodeikticus andClostridium perfringens were fragmented to about 7 000 nucleotide pairs long by shear and fractionated with respect to buoyant density of mercury complexes in Cs2SO4. The distribution of G + C content in both DNA's was characteristically asymmetric. InM. lysodeikticus DNA, low G + C fragments were more numerous than high G + C fragments, whereas inC. perfringens DNA, high G + C fragments were more numerous than low G + C fragments. The G + C content of fragments ofM. lysodeikticus DNA varied from 70 to 77%, with a mean and standard deviation of 73.7 ± 1.92% G + C and that ofC. perfringens DNA varied from 27 to 34%, with a mean and standard deviation of 29.8 ± 1.34% G + C. The standard deviation was smaller than that ofEscherichia coli DNA fragments of similar size. Biological meanings of relatively low heterogeneity in nucleotide composition inM. lysodeikticus andC. perfringens are discussed. |
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Keywords: | Micrococcus lysodeikticus Clostridium perfringens DNA Hg Cs2SO4 Low Heterogeneity Composition Bias at the Third Coding Positions tRNA |
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