Immunochemical detection of ochratoxin A in black Aspergillus strains |
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| Authors: | József Téren János Varga Zsuzsanna Hamari Edit Rinyu Ferenc Kevei |
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| Affiliation: | (1) Animal Health and Food Control Station, P.O. Box 446, H-6701 Szeged, Hungary;(2) Department of Microbiology, Attila József University, P.O. Box 533, H-6701 Szeged, Hungary |
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| Abstract: | One hundred and fifty-seven strains belonging to Aspergillus section Nigri were tested for ochratoxin A production using three different methods: a relatively new immunochemical method based on an enzyme-linked immunosorbent assay (ELISA), thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The monoclonal antibody-based ELISA technique was successfully used to screen for low levels of ochratoxin A in the black Aspergilli without concentrating the culture filtrates. The results were confirmed by TLC and HPLC analysis and chemical derivatization. These latter methods required concentrated filtrates. Ochratoxin A was detected in the culture filtrates of five of the 12 A. carbonarius strains, none of the 45 A.japonicus strains and three of the 100 isolates in the A. niger aggregate (A. foetidus, A. awamori and A. niger).Abbreviations ELISA enzyme-linked immunosorbent assay - HPLC high-performance liquid chromatography - OA ochratoxin A - TLC thin-layer chromatography |
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| Keywords: | Aspergillus awamori Aspergillus carbonarius Aspergillus foetidus Aspergillus niger ELISA ochratoxin A |
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