The neurotoxicological effects of mastoparan Polybia-MPII at the murine neuromuscular junction: an ultrastructural and immunocytochemical study |
| |
Authors: | Thalita Rocha Bibiana M de Souza Mario S Palma Maria Alice da Cruz-Höfling John Buchanan Harris |
| |
Institution: | 1.Department of Histology and Embryology,Institute of Biology, University of Campinas, UNICAMP,Campinas,Brazil;2.Department of Biology,Institute of Biosciences, Center of Studies of Social Insects, S?o Paulo State University, UNESP,Rio Claro,Brazil;3.Medical Toxicology Centre, Institute of Neuroscience, The Medical School,Newcastle University,Newcastle upon Tyne,UK |
| |
Abstract: | Polybia-MPII (INWLKLGKMVIDAL-NH2), a mastoparan isolated from the crude venom of the swarming wasp Polybia paulista, was injected into the left hind limb of Swiss white mice. Between 3 h and 21 days later the mice were killed and the soleus
muscles from both hind limbs were removed. Sections of the muscles were made for transmission electron microscopy and immunocytochemistry.
Transmission electron microscopy showed that both the volume fraction occupied by synaptic vesicles and synaptic vesicle density
was greatly reduced after exposure to Polybia-MPII, although there was no significant structural damage to the plasma membrane
of the terminal boutons and mitochondria were indistinguishable from those in normal, control boutons. Immunocytochemistry
revealed that in control muscles 99% of motor end plates identified by the positive labelling of acetylcholine receptors by
TRITC-α-bungarotoxin co-labelled with anti-synaptophysin antibody, but this figure fell by 30% in muscles exposed to the toxin.
These changes were transient. They were maximal at 6 h and fully reversed by 3 days. At no time was axonal labelling with
anti-neurofilament antibodies affected by exposure to Polybia-MPII. We conclude that mastoparan Polybia-MPII is a minor neurotoxin
and suggest that its neurotoxic activity is unlikely to be of clinical significance. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|