Improvement of welan gum production and redistribution of metabolic flux under pH control process in Alcaligenes sp. CGMCC2428 |
| |
Authors: | Hui Li Sha Li Hong Xu Xiaoye Chen Pingkai Ouyang |
| |
Institution: | 1423. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Engineering and Light Industry, Nanjing University of Technology, Nanjing, 210-009, China 2423. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214-122, China
|
| |
Abstract: | Batch fermentations of welan gum from Alcaligenes sp. CGMCC2428 at pH values of 5.5 ~ 7.0 were studied. Based on the kinetic analysis, a pH control process for improving welan production was developed. By maintaining the culture pH at 7.0, the process significantly improved the maximal welan concentration and productivity to reach 25.1 ± 0.65 g/L and 0.42 ± 0.003 g/L/h, respectively, compared with those in native pH processes where pH value would decrease from 7.0 to 5.1 (18.5 ± 0.72 g/L and 0.28 ± 0.002 g/L/h). This improvement may be due to the increased metabolic flux of glucose-1-phosphate to welan induced by pH control process. Furthermore, scale-up fermentation under controlled pH was implemented using 300-L fermentor. The highest welan concentration of 27.5 ± 0.97 g/L was obtained. This simplified process proved effective in industry fermentation for high welan production. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|