Effect of a bifunctional imidoester on dissociation of 70S ribosomes in Escherichia coli |
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Authors: | Y Nakamura A Wada |
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Affiliation: | Department of Physics, Kyoto University, Kyoto, Japan |
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Abstract: | Treatment of the 70S ribosome from with diethyl malonimidate dihydrochloride, a bifunctional imidoester, was found to result in the formation of crosslinkage between the two subunits. The 70S complex thus obtained no longer dissociates into 50S and 30S particles at 0.5mM Mg2+ concentration, but do so at lower concentrations (0.1mM), suggesting the release of protein(s) involved in the inter-particle cross-linkage from one or both ribosomal subunits. |
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Keywords: | To whom correspondence should be addressed. Present address: Institute for Virus Research Kyoto University Kyoto Japan |
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