Identification and partial purification of a human natural killer cell proliferation-inducing factor |
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Authors: | Queen B Saxena Rajiv K Saxena Ronald H Goldfarb Ronald B Herberman Theresa L Whiteside |
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Institution: | (1) Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 15213 Pittsburgh, PA, USA;(2) School of Life Sciences, Jawaharlal Nehru University, 110067 New Delhi, India |
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Abstract: | Culture supernatants of Concanavalin A activated human peripheral blood mononuclear cells were found to contain a factor which
induced proliferative response in normal peripheral blood mononuclear cells. This proliferation-inducing factor specifically
induced and sustained proliferation of purified human NK cells but not of T or B cells. Although interleukin 2 (IL12) also
has proliferation-inducing effects on NK cells, the partially purified proliferationinducing factor preparations contained
no measurable IL2 contamination. Moreover, neutralizing anti-IL2 antibodies did not block the growth effect of proliferation-inducing
factor on purified human NK cells. Other cytokines which were tested, including IL4, IL6, IL7, IL12, TNF and IFN, were all
found to be inactive in the proliferation-inducing factor assay. While proliferation-inducing factor by itself had no effect
on T-cell proliferation, IL2-induced proliferation of T cells was significantly enhanced in the presence of proliferation-inducing
factor, as was IL2-induced NK-cell proliferation. NK cells could be maintained in culture for at least a month in the presence
of proliferation-inducing factor alone, but the cells lost their cytolytic activity after 3–4 weeks in culture. Addition of
IL2, to NK cells which had been cultured in the presence of proliferation-inducing factor, restored their cytotoxicity. Proliferation-inducing
factor activity was partially purified on an anion exchange HPLC column. The molecular weight of proliferation-inducing factor
appeared to be about 10 kDa, based on its elution profile on a sizing HPLC column. Our results indicate that proliferation-inducing
factor is a novel NK-cell proliferationinducing factor. |
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Keywords: | NK cells mitogen cytokines interleukin MHC-AF protein purification |
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