利用SSR定位籼稻品种Kaharamana中抗褐飞虱基因Bph9

褐飞虱是危害水稻生产最重要的害虫之一，利用寄主抗性被认为是防治褐飞虱最经济而有效的方法。斯里兰卡水稻品种Kaharamana对东亚和东南亚的褐飞虱种群均表现抗虫性，利用分子遗传学的方法对其携带的Bph9基因进行了SSR定位。所用的遗传群体为来源于Kaharamana和02428的含有180个单株的F2分离群体，每个F2单株套袋自交获得F2：3家系。利用苗期集团鉴定埘F2：3家系进行抗褐飞虱鉴定，以推测相应F2单株的基因型。连锁分析表明，Bph9位于第12染色体上的两个SSR标记RM463和RM5341之间，分别与之相距6．8cM和9．7cM。该标记有助十将Bph9用于分子标记辅助选择育种研究。

SSR Mapping of Brown Planthopper Resistance Gene Bph9 in Kaharamana, an Indica Rice (Oryza sativa L.)

The brown planthopper (BPH) is one of the most serious insects pests of rice, and the host resistance has been recognized as one of the most economic and effective measures for BPH management. In this study, we conducted a molecular-based genetic analysis of Bph9 in Kaharamana, a Sri Lanka rice variety resistant to BPH insects of East and Southeast Asia. An F2 segregating population composed of 180 plants was constructed from the cross between Kaharamana and 02428, and each F2 plant was self-crossed to obtain F2:3 family. The bulked seedling test method was used to evaluate the resistance of F2:3 families, and the genotype of each F2 plant was inferred from the phenotype of corresponding F2 3 family. Linkage analysis indicated that the resistant gene Bph9 in Kaharamana was located between SSR markers RM463 and RM5341 on chromosome 12 with linkage distances of 6.8 cM and 9.7 cM, respectively. The time- and money-saving SSR markers would be helpful in the application of Bph9 in breeding program via marker-assisted selection.