锈毛草莓积雪草合剂对STZ小鼠降血糖作用研究

采用75%乙醇萃取锈毛草莓及积雪草,制备成合剂,通过STZ诱导建立糖尿病小鼠模型,随机分为模型组、低剂量组及高剂量组,分别以蒸馏水、4及8 g·kg~(-1)锈毛草莓积雪草合剂灌胃,1次·d~(-1),持续4周,另设正常组作为对照。探究绣毛草莓积雪草合剂对糖尿病小鼠的降血糖效果及作用机制。结果表明:(1)不同剂量的锈毛草莓积雪草合剂均能显著降低糖尿小鼠血糖值,提高糖耐量并减轻其多食、多饮、多尿的症状。(2)低剂量组小鼠血清TC、TG含量较模型组有显著提高(P0.01或P0.05),肝脏SOD、CAT活性显著提高(P0.05),MDA含量明显降低。(3)低剂量黄毛草莓积雪草合剂效果优于二甲双胍,毒性实验显示锈毛草莓积雪草合剂实属无毒。该研究结果表明锈毛草莓积雪草合剂具有降血糖作用,同时能够降低糖尿病小鼠的血脂水平,其作用机制可能与提高肝脏抗氧化能力有关。

Antidiabetic effects of Fragaria nilgerrensis and Centella asiatica compound on STZ-induced diabetic mice

We extracted medicine compound(FN-MC)by 75% ethanol. STZ-induced diabetic mice were randomly divided into model, low-dose and high-dose groups, respectively, and treated with distilled water and FN-MC at doses of 4 and 8 g·kg^-1 for 4 weeks. Control group was treated with distilled water. The results showed that FN-MC could significantly lower the fasting blood glucose, reduced food and water intake, and improved the level of OGGT. Low-dose FN-MC could obviously increase the levels of TC and TG(P<0.01 or P<0.05), decrease the level of MDA, enhance the activities of SOD and CAT(P<0.05). Besides, Low-dose FN-MC was more effective than metformin. Toxicity test showed that FN-MC was non-toxic. In conclusion, FN-MC could decrease blood glucose in STZ-induced diabetic mice by mechanisms of enhancing the activity of antioxidant in liver.