番茄皂苷A对小鼠血脂及肝脏脂肪的作用

该研究以ApoE基因缺陷小鼠和高脂饲料诱导的高血脂症模型小鼠为对象,采用药理学方法研究了番茄皂苷A对血脂及肝脏脂肪的调节作用。在ApoE基因缺陷小鼠和高脂饲料诱导的高血脂症模型小鼠中,通过灌胃给予番茄皂苷A:取血,测定血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素氮(BUN)、肌酐(Cr)、葡萄糖(Glu)的含量和活性;处死小鼠后,取肝脏称重,计算肝脏指数;精确称取一部分肝脏,测定肝脏脂质的含量。结果表明:番茄皂苷A对ApoE基因缺陷小鼠可以降低血清TC、HDLC、LDLC的含量,对ALT、AST、BUN、Cr、Glu没有影响,说明番茄皂苷A可以降低ApoE基因缺陷小鼠血中胆固醇含量,对血糖没有影响,对肝肾功能无影响;对高脂饲料诱导的高血脂症模型小鼠,可以降低血清TC、HDLC的含量,可以降低肝脏TC的含量,对ALT、AST、BUN、Cr、Glu没有影响,说明番茄皂苷A可以改善高脂饲料诱导的高血脂症模型小鼠的脂质代谢,且对肝肾功能无影响。该研究结果表明番茄皂苷A具有一定的降低胆固醇的作用,且不影响肝肾功能。

Effects of esculeoside A from Lycopersicon esculentum on serum lipid and liver lipid in mice

Tomato saponin crude extract is helpful for preventing hyperlipemia. Esculeoside A is the main saponin compound in tomato saponin crude extract. We investigated the effects of esculeoside A on modulating levels of serum lipid, liver lipid in ApoE-deficient mice and experimental hyperlipidemia mice. Esculeoside A was administrated at a dose of 50 mg·kg^-1 and 100 mg·kg^-1 daily by gavage for 60 d to ApoE-deficient mice and experimental hyperlipidemia mice. We measured leves of total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDLC), low density lipoprotein cholesterol(LDLC), alanine aminotransferases(ALT), aspartate aminotransferases(AST), blood urea nitrogen(BUN), creatinine(Cr), glucose(Gru)in serum. The liver of each mouse was extracted, weighted and the li-ver indexes were calculated. Liver TC and TG levels were determined using a semi-automatic biochemical analyzer according to the method described in the tissue TC and TG commercial kit. The results showed that esculeoside A had no effect on levels of ALT, AST, BUN, Cr and Gru, but decreased TC, HDLC, LDLC levels in serum in ApoE-deficient mice, and had no effect on hepatic and renal functions. Esculeoside A could decreased TC and HDLC levels in serum and decreased TC levels in liver in experimental hyperlipidemia mice, and had no effect on hepatic and renal functions. It is indicated that esculeoside A can improve lipid metabolism in ApoE-deficient mice and experimental hyperlipidemia mice, and has no effect on hepatic and renal functions.