| 棕色固氮菌含铁超氧化物歧化酶的分离提纯及性质研究 |
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| 引用本文： | 罗贵民,李惟,陈惠鹏,张岩,程玉华. 棕色固氮菌含铁超氧化物歧化酶的分离提纯及性质研究[J]. 中国生物化学与分子生物学报, 1988, 4(3): 193-200 |
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| 作者姓名： | 罗贵民 李惟 陈惠鹏 张岩 程玉华 |
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| 作者单位： | 古林大学酶工程实验室，古林大学生物学系，古林大学酶工程实验室，古林大学酶工程实验室，古林大学酶工程实验室 长春，长春，长春，长春 |
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| 摘 要： | 本文以改进的方法提纯了棕色固氮菌——230含铁超氧化物歧化酶。产品收率提高1倍以上。该酶在pH8.2时,5℃～60℃稳定。在25℃时,pH6～12稳定。NaN_3和H_2O_2是该酶的抑制剂,而KCN对此酶活力无影响。
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| 关 键 词： | 棕色固氮菌 超氧化物歧化酶 稳定性 抑制剂 |
| 收稿时间： | 1988-06-20 |
PURIFICATION AND SOME PROPERTIES OF IRON-CONTAINING SUPEROXIDE DISMUTASE FROM AZOTOBACTER VLNEIANDII |
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| Luo,Gui-Min Li,Wei Chen,Hui-Peng Zhang,Yan Cheng,Yu-Hua. PURIFICATION AND SOME PROPERTIES OF IRON-CONTAINING SUPEROXIDE DISMUTASE FROM AZOTOBACTER VLNEIANDII[J]. Chinese Journal of Biochemistry and Molecular Biology, 1988, 4(3): 193-200 |
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| Authors: | Luo Gui-Min Li Wei Chen Hui-Peng Zhang Yan Cheng Yu-Hua |
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| Affiliation: | (The Lab of Enzyme Enyineering, Jilin University, Chang Chun |
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| Abstract: | An improved method of isolation and purification of iron-containing superoxide dismutase from Azotobacter vinelandii- 230 has been developed. Using this method the yield of the enzyme activity is double, and the physical properties of the isolated enzyme are similar to that obtained previously. The enzyme is stable at 5-60℃, pH8.2 and 25℃, pH 6-12. The enzyme activity can be inhibited by NaN3 and H2O2, and unaffected by KCN. |
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| Keywords: | Superoxide Dismutase Azotobacter vinelandii Stability Inhibitors. |
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