Studies on tryptophan synthetases from various strains of Bacillus subtilis |
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| Authors: | Jerzy W Meduski and Stephen Zamenhof |
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| Institution: | Department of Medical Microbiology and Immunology, Department of Biological Chemistry, and Molecular Biology Institute, University of California at Los Angeles, Calif. 90024, U.S.A. |
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| Abstract: | 1. Tryptophan synthetase B of three strains of Bacillus subtilis was prepared from ;exo-protoplastic' and ;endo-protoplastic' fractions; the enzyme from ;exo-protoplastic' fraction was purified 30- to 120-fold by ammonium sulphate precipitation and DEAE-cellulose column chromatography; the latter step separated this enzyme from tryptophan synthetase A, tryptophanase and proteolytic enzymes, but the purified preparations were not stable. 2. The activity of tryptophan synthetase B did not depend on the presence of tryptophan synthetase A. 3. Tryptophan synthetases B of the strains tested differed in their utilization of 2- and 7-methylindole as compared with indole; this suggests that these tryptophan synthetases B are not identical. |
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