The Effect of L-Phenylalanine on Phenylalanine Ammonia-lyase and Cell Division in Artichoke Callus Culture |
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Authors: | JAMES, D. J DAVIDSON, A. W. TRUEMAN, M. J. |
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Affiliation: | School of Biological Sciences, Thames Polytechnic Wellington Street, London, S.E 18 |
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Abstract: | 5 x 105 M L-phenylalanine overcame the inhibitory effectof white light on cell division in artichoke callus culturesand increased extractable phenylalanine ammonia-lyase (PAL)activity compared to cultures grown in the presence of 5 x 104M phenylalanine The lower concentration of the amino acid alsoenhanced rates of uptake and incorporation of 14C labelled phenylalaninethroughout G1 and S. Differences between the two concentrationswere greatest during S with a 4-fold increase in uptake anda 3-fold increase in incorporation It is suggested thereforethat the capacity of 5 x105 M phenylalanine to offsetthe light effect is due to an indirect stimulatory effect onamino acid and protein metabolism Increased levels of extractablePAL activity would then be reflected by this general stimulationof protein synthesis. Helianthus tuberosus L, Jerusalem artichoke, callus culture, cell division, phenylalanine ammonia-lyase |
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