Identification of the orphan G protein-coupled receptor GPR31 as a receptor for 12-(S)-hydroxyeicosatetraenoic acid |
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Authors: | Guo Yande Zhang Wenliang Giroux Craig Cai Yinlong Ekambaram Prasanna Dilly Ashok-Kumar Hsu Andrew Zhou Senlin Maddipati Krishna Rao Liu Jingjing Joshi Sangeeta Tucker Stephanie C Lee Menq-Jer Honn Kenneth V |
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Affiliation: | Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48202, USA. |
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Abstract: | Hydroxy fatty acids are critical lipid mediators involved in various pathophysiologic functions. We cloned and identified GPR31, a plasma membrane orphan G protein-coupled receptor that displays high affinity for the human 12-lipoxygenase-derived product 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE). Thus, GPR31 is named 12-(S)-HETE receptor (12-HETER) in this study. The cloned 12-HETER demonstrated high affinity binding for 12-(S)-[(3)H]HETE (K(d) = 4.8 ± 0.12 nm). Also, 12-(S)-HETE efficiently and selectively stimulated GTPγS coupling in the membranes of 12-HETER-transfected cells (EC(50) = 0.28 ± 1.26 nm). Activating GTPγS coupling with 12-(S)-HETE proved to be both regio- and stereospecific. Also, 12-(S)-HETE/12-HETER interactions lead to activation of ERK1/2, MEK, and NFκB. Moreover, knocking down 12-HRTER specifically inhibited 12-(S)-HETE-stimulated cell invasion. Thus, 12-HETER represents the first identified high affinity receptor for the 12-(S)-HETE hydroxyl fatty acids. |
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Keywords: | Eicosanoid Eicosanoid Receptor G Protein-coupled Receptors (GPCR) Receptors Signal Transduction |
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