Localization of proteasomes in plant cells |
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Authors: | Ulrike Vallon U Kull |
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Institution: | (1) Biologisches Institut der Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany |
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Abstract: | Summary Proteasomes, also known as multicatalytic proteinase complexes, were localized in suspension cells of potato (Solanum tuberosum) by direct immunofluorescence using polyclonal antibodies labelled with fluorescein isothiocyanate. The method used allows an estimate of relative amounts of proteasomal antigens in different cell components. Proteasomes are present in the nuclei and the cytoplasm. The nucleoplasm contains small areas of weak fluorescence. The peripheral cytoplasm and possibly elements of the cytoskeleton show higher fluorescence than other parts of the cytoplasm. This indicates a localization of proteasomes similar to that known from animal cells.Abbreviations DMSO
dimethylsulfoxide
- EGTA
ethyleneglycol-bis-( -aminoethylether)-N,N,N ,N -tetra acetic acid
- FITC
fluorescein isothiocyanate
- PBS
phosphate buffered saline
- PIPES
piperazine-1,4-bis-(2-ethanesulfonic acid) |
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Keywords: | Cytoskeleton Immunofluorescence Multicatalytic proteinase Nucleus Plant cell Proteasome |
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