Spatio-temporal kinematic analysis of shoot gravitropism in Arabidopsis thaliana |
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Authors: | Satoru Tsugawa Norihiro Kanda Moritaka Nakamura Tatsuaki Goh Misato Ohtani Taku Demura |
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Institution: | 1.Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan;2.Division of Plant Environmental Responses, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan;3.Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan |
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Abstract: | Plant shoots can bend upward against gravity, a behavior known as shoot gravitropism. The conventional quantification of shoot bending has been restricted to measurements of shoot tip angle, which cannot fully describe the spatio-temporal bending process. Recently, however, advanced imaging analyses have been developed to quantify in detail the spatio-temporal changes in inclination angle and curvature of the shoot. We used one such method (KymoRod) to analyze the gravitropism of the Arabidopsis thaliana inflorescence stem, and successfully extracted characteristics that capture when and where bending occurs. Furthermore, we implemented an elastic spring theoretical model and successfully determined best fitted parameters that may explain typical bending behaviors of the inflorescence stem. Overall, we propose a data-model combined framework to quantitatively investigate shoot gravitropism in plants. |
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Keywords: | kinematics mathematical model proprioception shoot gravitropism |
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