Human plasma gelsolin binds adenosine triphosphate |
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| Authors: | H Yamamoto H Ito H Nakamura E Hayashi S Kishimoto T Hashimoto K Tagawa |
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| Institution: | Third Department of Internal Medicine, Osaka University School of Medicine. |
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| Abstract: | Binding studies of human plasma gelsolin with ATP were done by equilibrium dialysis. Analysis of the binding data showed that plasma gelsolin had one class of ATP binding site with Kd = 2.8 x 10(-7) M, which saturated at an ATP/gelsolin ratio of 0.6. The bioluminescent assay for ATP with luciferin and firefly luciferase confirmed that the protein contained a nucleotide as ATP. |
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