Proteome analysis of rat liver mitochondria reveals a possible compensatory response to endotoxic shock |
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| Authors: | Miller Ingrid Gemeiner Manfred Gesslbauer Bernd Kungl Andreas Piskernik Christina Haindl Susanne Nürnberger Silvia Bahrami Soheyl Redl Heinz Kozlov Andrey V |
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| Affiliation: | Institute of Medical Chemistry, Department of Natural Sciences, University of Veterinary Medicine Vienna, Austria. |
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| Abstract: | Organ failure induced by endotoxic shock has recently been associated with affected mitochondrial function. In this study, effects of in vivo lipopolysaccharide-challenge on protein patterns of rat liver mitochondria in treated animals versus controls were studied by two-dimensional electrophoresis (differential image gel electrophoresis). Significant upregulation was found for ATP-synthase alpha chain and superoxide dismutase [Mn]. Our data suggest that endotoxic shock mediated changes in the mitochondrial proteome contribute to a compensatory reaction (adaptation to endotoxic shock) rather than to a mechanism of cell damage. |
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| Keywords: | 2-DE, two-dimensional electrophoresis ALDH1, mitochondrial aldehyde dehydrogenase ALT, alanine aminotransferase DIGE, differential image gel electrophoresis hsp60, 60 kD heat shock protein LPS, lipopolysaccharide MS, mass spectrometry ROS, reactive oxygen species SOD, superoxide dismutase |
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