Differential appearance of dynamin in constitutive and regulated exo-endocytosis: a single-cell multiplex RT-PCR study |
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Authors: | Email author" target="_blank">Detlev?GrabsEmail author Mathias?Bergmann |
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Institution: | (1) Department of Medicine/Anatomy, University Fribourg, Rte A. Gockel 1, 1700 Fribourg, Switzerland |
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Abstract: | Neurons in the central nervous system establish, via their axons and dendrites, an extended network that allows synaptic transmission.
During developmental maturation and process outgrowth, membrane turnover is necessary for the enlargement and subsequent growth
of axons and dendrites from the perikarya to the target cell (constitutive exocytosis/endocytosis). After targeting and synapse
formation, small synaptic vesicles are needed for the quantal release of neurotransmitters from the presynaptic terminal with
subsequent recycling by regulated exocytosis/endocytosis. An investigation of the onset of the appearance of mRNA and protein
in dissociated cultures of neurons from mouse hippocampus or from chick retina has shown an early abundance of proteins involved
in exocytosis, such as syntaxin 1, SNAP-25, and synaptotagmin 1, whereas dynamin 1, a protein necessary for clathrin-mediated
endocytosis, can be detected only after neurons have established contacts with neighboring cells. The results reveal that
constitutive membrane incorporation and regulated synaptic transmitter release is mediated by the same neuronal proteins.
Moreover, the data exclude that dynamin 1 takes part in constitutive recycling before synapse formation, but dynamin 2 is
present at this stage. Thus, dynamin 2 may be the constitutive counterpart of dynamin 1 in growing neurons. Synapse establishment
is linked to an upregulation of dynamin 1 and thereby represents the beginning of the regulated recycling of membranes back
into the presynaptic terminal. |
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Keywords: | Neuronal development Exocytosis Endocytosis Presynaptic proteins Dynamin Chick (White Leghorn) Mouse |
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