A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations |
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| Authors: | John S Beck Anne E Kwitek Phillip H Cogen Andrew K Metzger Geoffrey M Duyk Val C Sheffield |
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| Institution: | (1) Division of Medical Genetics, Department of Pediatrics 2615 JCP, University of Iowa Hospitals, 52242 Iowa City, IA, USA;(2) Brain Tumor Research Center, Department of Neurological Surgery, School of Medicine, University of California, San Francisco, California, USA;(3) Department of Genetics and the Howard Hughes Medical Institute, Harvard University, Boston, Massachusetts, USA |
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| Abstract: | p53 is a tumor suppressor gene located on 17p, a region of the human genome frequently deleted in tumors. Mutation of the p53 gene is an important step leading to development of many forms of human cancer. To simplify the analysis of tumors for p53 point mutations, we describe a GC-clamped denaturing gradient gel assay for detecting single-base substitutions within highly conserved regions of the p53 gene. This assay alows for efficient screening of tumors for single-base substitutions within the p53 gene and can be used to facilitate sequence analysis of p53 point mutations. |
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