Transfer RNA species in normal and leukemic human lymphoblasts |
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| Authors: | R C Gallo S Pestka |
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| Affiliation: | 1. Laboratory for Molecular Sensing, Institute of Protein Biochemistry–National Research Council (IBP–CNR), 80131 Naples, Italy;2. Institute of Food Sciences–National Research Council (ISA–CNR), 83100 Avellino, Italy;1. Key Laboratory of Genomics and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China;4. Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China;1. Department of Biochemistry, School of Medicine, University of Patras, 26504 Patras, Greece;2. Laboratory of Public Health, School of Medicine, University of Patras, 26504 Patras, Greece;1. Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China;1. Division of Oncology, Hematology and Infectious Diseases, Department of Internal Medicine, Fukuoka University, Fukuoka, Japan;1. Departamento de Fisiología, Genética y Microbiología, Facultad de Ciencias, Universidad de Alicante, 03080 Alicante, Spain;2. Instituto Multidisciplinar para el Estudio del Medio “Ramón Margalef” (IMEM), Universidad de Alicante, 03080 Alicante, Spain;3. Neurociencia Clínica y Experimental (NiCE), Facultad de Medicina, Instituto de Investigación en Envejecimiento, Instituto Murciano de Investigación Biosanitaria (IMIB), Universidad de Murcia, 30071 Murcia, Spain |
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| Abstract: | The results of a study to identify the transfer RNA's for every amino acid in human tissues are reported. The isoaccepting species of the tRNA of human lymphoblasts (normal and leukemic) were fractionated by reverse-phase partition column chromatography. In every case, comparisons were made between the aminoacyl-tRNA profile of normal and leukemic lymphoblasts by co-chromatography using 3H for one label and 14C for another and the homologous amino acid-activating enzyme. Differences between the profiles of normal and leukemic cells were re-evaluated by reversal of the labeled amino acid and by acylation with the heterologous enzyme. The results indicate that (1) there are at least 56 species of tRNA fractionated from both the normal and the leukemic cells; (2) for most cases the profiles of the leukemic cells were very similar if not identical to the profiles of normal cells; (3) small but reproducible differences were found for leucyl-, seryl-, threonyl- and prolyl-tRNA; (4) the most pronounced differences were with tyrosyl-tRNA and glutaminyl-tRNA. The difference in the glutaminyl-tRNA was an extra isoaccepting species found only when normal lymphoblast enzyme preparation was used. Therefore, in this case the difference was due to the enzyme preparation rather than the tRNA itself. |
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