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Phenylalanine ammonia lyase fromSporidiobolus pararoseus andRhodosporidium toruloides: Application for phenylalanine and tyrosine deamination |
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| Authors: | S. Kawasaki Watanabe G. Hernandez-Velazco F. Iturbe-Chiñas A. Lopez-Munguia |
| Affiliation: | (1) the Departmento de Alimentos y Biotecnologia, Facultad de Quimica, UNAM, Cd. Universitaria, 04510 Mexico City, Mexico;(2) the Instituto de Biotecnologia, UNAM, Apartado Postal 510-3, 62271 Cuernavaca, Morelos, Mexico |
| Abstract: | Simultaneous depletion of phenylalanine and tyrosine by phenylalanine ammonia lyase is described in a mutual competitive inhibition model. The enzymes obtained fromSporidiobolus pararoseus andRhodosporidium toruloides were charaterized in terms of stability, optimal reaction parameters and kinetic behaviour. Both enzymes followed Michaelis-Menten kinetics with respect to the two amino acids. However, the enzyme fromRhodosporidium toruloides was inhibited by high tyrosine concentrations. |
| Keywords: | Deamination phenylalanine ammonia lyase phenylketonuria Rhodosporidium toruloides Sporidiobolus pararoseus |
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