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The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2 |
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| Institution: | 1. UMR INRAE 0085, CNRS 7247, Physiologie de la Reproduction et des Comportements, Centre INRA Val de Loire, 37380 Nouzilly, France;2. UMR MEPHI D-258, I, IRD, Aix Marseille Université, 19-21 Boulevard Jean Moulin, 13005 Marseille, France;3. CNRS SNC 5039, 13005 Marseille, France;4. EA GICC 7501, CHRU de Tours, 37044 TOURS Cedex 09, France;5. Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA;6. Weill Cornell Medical College, Cornell University, New York, NY, USA;7. Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA;1. School of Medicine, Queen’s University, ON, Canada;2. Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, United States;1. Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany;2. Division of Pediatric Hematology/Oncology, Boston Children''s Hospital, Harvard Medical School, Boston, Massachusetts, USA;3. REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany;1. Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA;2. Center for RNA Science and Therapeutics, Case Western Reserve University, Cleveland, OH 44106, USA;3. Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA;4. The Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106, USA;5. Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA;1. Department of Biochemistry and Molecular Biology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, 300070 Tianjin, PR China;2. CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, PR China |
| Abstract: | The vertebrate piggyBac derived transposase 5 (PGBD5) encodes a domesticated transposase, which is active and able to transpose its distantly related piggyBac-like element (pble), Ifp2. This raised the question whether PGBD5 would be more effective at mobilizing a phylogenetically closely related pble element. We aimed to identify the pble most closely related to the pgbd5 gene. We updated the landscape of vertebrate pgbd genes to develop efficient filters and identify the most closely related pble to each of these genes. We found that Tcr-pble is phylogenetically the closest pble to the pgbd5 gene. Furthermore, we evaluated the capacity of two murine and human PGBD5 isoforms, Mm523 and Hs524, to transpose both Tcr-pble and Ifp2 elements. We found that both pbles could be transposed by Mm523 with similar efficiency. However, integrations of both pbles occurred through both proper transposition and improper PGBD5-dependent recombination. This suggested that the ability of PGBD5 to bind both pbles may not be based on the primary sequence of element ends, but may involve recognition of inner DNA motifs, possibly related to palindromic repeats. In agreement with this hypothesis, we identified internal palindromic repeats near the end of 24 pble sequences, which display distinct sequences. |
| Keywords: | domestication transposition DNA binding molecular evolution transposable element CMV"} {"#name":"keyword" "$":{"id":"k0035"} "$$":[{"#name":"text" "_":"Human cytomegalovirus CRD"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"Cystein-rich domain GFP"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "_":"green fluorescent protein LTR"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"long terminal repeats NeoR"} {"#name":"keyword" "$":{"id":"k0075"} "$$":[{"#name":"text" "_":"Neomycin resistance ORF"} {"#name":"keyword" "$":{"id":"k0085"} "$$":[{"#name":"text" "_":"Open reading frame STIR"} {"#name":"keyword" "$":{"id":"k0115"} "$$":[{"#name":"text" "_":"sub-terminal inverted repeats SV40"} {"#name":"keyword" "$":{"id":"k0125"} "$$":[{"#name":"text" "_":"simian virus 40 TE"} {"#name":"keyword" "$":{"id":"k0135"} "$$":[{"#name":"text" "_":"transposable element TIR"} {"#name":"keyword" "$":{"id":"k0145"} "$$":[{"#name":"text" "_":"terminal inverted repeats TSD"} {"#name":"keyword" "$":{"id":"k0155"} "$$":[{"#name":"text" "_":"target site duplication |
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