Fat accumulation in differentiated brown adipocytes is linked with expression of Hox genes |
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| Affiliation: | 1. Animal Biochemistry Division, National Dairy Researikch Institute, Karnal, Haryana, 132001, India;2. Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana, 132001, India;3. Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, 132001, India;1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;1. National Dairy Research Institute, Animal Biotechnology Centre, Karnal 132001, India;2. Department of Biochemistry, Banaras Hindu University, 221005 Varanasi, India;1. Hospices civils de Lyon, centre hospitalier Lyon Sud, service de biochimie biologie moléculaire Sud, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France;2. Université Lyon 1, faculté de médecine Lyon Est, centre Léon-Bérard, centre de recherche en cancérologie de Lyon, Inserm 1052 CNRS 5286, 69373 Lyon cedex 08, France;3. CHRU de Lille, hôpital Huriez, service d’endocrinologie et métabolisme, 59000 Lille, France;4. Université de Lyon, CNRS, Inra, université Claude-Bernard Lyon 1, École normale supérieure de Lyon, Institut de génomique fonctionnelle de Lyon, 69007 Lyon, France;1. Second Department of Cardiology, Laboratory of Cardiac Electrophysiology, "Evangelismos" General Hospital of Athens, Athens, Greece;2. First Department of Cardiology, University of Ioannina, Medical School, Ioannina, Greece;3. Department of Cardiology, Helena Venizelou Hospital, Athens, Greece;1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;2. Department of Veterinary Management of Animal Resources, Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, Liège, Belgium;3. Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100000, China;1. Department of Internal Medicine, Valme University Hospital, UGC Internal Medicine, Servicio Andaluz de Salud, Seville E-41014, Spain;2. Department of Digestive Diseases, Valme University Hospital, UGC Digestive Diseases, Servicio Andaluz de Salud, Seville E-41014, Spain |
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| Abstract: | Homeobox (Hox) genes are involved in body plan of embryo along the anterior–posterior axis. Presence of several Hox genes in white adipose tissue (WAT) and brown adipose tissue (BAT) is indicative of involvement of Hox genes in adipogenesis. We propose that differentiation inducing agents viz. isobutyl-methyl-xanthine (IBMX), indomethacin, dexamethasone (DEX), triiodothyronine (T3) and insulin may regulate differentiation in brown adipose tissue through Hox genes. In vitro culture of brown fat stromalvascular fraction (SVF) in presence or absence of differentiation inducing agents was used for establishing relationship between fat accumulation in differentiated adipocytes and expression of Hox genes. Relative expression of Pref1, UCP1 and Hox genes was determined in different stages of adipogenesis. Presence or absence of IBMX, indomethacin and DEX during differentiation of proliferated pre-adipocytes resulted in marked differences in expression of Hox genes and lipid accumulation. In presence of these inducing agents, lipid accumulation as well as expression of HoxA1, HoxA5, HoxC4 & HoxC8 markedly enhanced. Irrespective of presence or absence of T3, insulin down regulates HoxA10. T3 results in over expression of HoxA5, HoxC4 and HoxC8 genes, whereas insulin up regulates expression of only HoxC8. Findings suggest that accumulation of fat in differentiated adipocytes is linked with expression of Hox genes. |
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| Keywords: | Brown fat differentiation Adipogenesis Insulin Triiodothyronine Induction medium BAT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" Brown adipose tissue CT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" Critical threshold DEX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" Dexamethasone IBMX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" Isobutyl-methyl-xanthine SVF" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" Stromalvascular fraction T3" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" Triiodothyronine UCP1" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" Uncoupling protein 1 WAT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" White adipose tissue |
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