Olfr603, an orphan olfactory receptor,is expressed in multiple specific embryonic tissues |
| |
| Affiliation: | 1. Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC;2. Department of Radiology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC;3. Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan, ROC;4. Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC;5. Institute of Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan, ROC;6. Department of Neurosurgery, Buddhist Tzu Chi General Hospital, Tzu Chi University, Hualien, Taiwan, ROC;7. Neuro-Medical Scientific Center, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC;1. Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China;2. Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China;1. Pharmacology and Toxicology and Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA;2. Environmental Health Science, New York Medical College, Valhalla, NY, USA;3. Environmental and Occupational Medicine, Rutgers University-Robert Wood Johnson Medical School, Piscataway, NJ, USA |
| |
| Abstract: | BackgroundOlfactory receptors were initially believed to be expressed specifically within the olfactory neurons. However, accumulating genome-scale data has recently demonstrated more extensive expression. There are hundreds of olfactory receptor family members and the realisation of their widespread expression provides an opportunity to reveal new biology. However, existing data is predominantly based on RT-PCR, microarray and RNA-seq approaches and the details of tissue and cell-type specific expression are lacking.ResultsAs a proof of principle, we selected Olfr603 for expression analysis. We generated an antibody against a non-conserved epitope of Olfr603 and characterised its expression in E8.5-E12.5 mouse embryos using immunohistochemistry. This analysis demonstrated a dynamic pattern of expression in diverse cell types within the developing embryo unrelated to the olfactory system. Expression was detected in migrating neural crest, endothelial precursors and vascular endothelium, endocardial cells, smooth muscle, neuroepithelium and within the ocular tissues. This complex distribution does not conform to any apparent germ layer or tissue origin.ConclusionsThis initial characterisation of Olfr603 expression highlights the potential for a broad role for this receptor in the development of many tissues. |
| |
| Keywords: | Olfactory receptor Embryonic expression Non-olfactory |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
