Interaction of gentamicin with phosphatidylserine/phosphatidylcholine mixtures in adsorption monolayers and thin liquid films: morphology and thermodynamic properties |
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Authors: | Georgi D Georgiev Georgi As Georgiev Z Lalchev |
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Institution: | (1) Faculty of Biology, Department of Biochemistry, University of Sofia, 8 Dragan Tsankov Str., 1164 Sofia, Bulgaria;; |
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Abstract: | Gentamicin possesses strong adverse actions like oto and nephrotoxicity. The latter is a result of strong gentamicin–acid
phospholipid interactions, resulting in cell fusion, fission, etc., ions as calcium interact with gentamicin and effectively
deter its toxicity. In this work, the interactions of gentamicin and Ca2+ with phosphatidylserine/phosphatidylcholine (PS/PC) mixtures of different ratio are experimentally characterized. Special
attention is paid to bridge thermodynamic and morphological properties of adsorption monolayers and thin liquid films (TLFs)
composed of these lipid mixtures. Our results show that gentamicin decreases the stability of common black TLFs formed of
pure PS coupled with suppression of lipid surface adsorption to the monolayers at the air–water interface; also, gentamicin
reveals effects of lowering of lipid spreading on the interface and significant loss of material during monolayer cycling,
increase of condensed phase, and organization of dense net-like domain monolayer texture. Gentamicin addition results in opposite
effects for films formed of DPPC/PS (95:5) mixture. It increases the stability of Newton black TLFs formed by DPPC/PS correlated
with faster and stronger surface adsorption and better surface spreading; also, gentamicin lowers the amount of condensed
phase and organization of domains of smaller size. We also showed that Ca2+ itself decreases the stability of common black TLFs formed of PS accompanied with weaker surface adsorption, formation of
higher amounts of condensed phase and organization of domains. In our experiments, Ca2+ softens, even deters, the effects of gentamicin on both PS and DPPC/PS films. |
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