Characterization of <Emphasis Type="Italic">Bradyrhizobium</Emphasis> Strains Isolated from Different Varieties of Soybean with 16SrDNA RFLP from Agricultural Land of Madhya Pradesh,India |
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Authors: | Anupama Bikrol Nidhi Saxena Kiran Singh |
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Institution: | (1) Department of Microbiology, Barkatullah University, Madhya Pradesh, Bhopal, 462026, India; |
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Abstract: | Madhya Pradesh is the major soybean contributor in India. The taxonomy of nitrogen fixing bacteria forming symbiotic associations
with leguminous plants has been deeply changed in recent years. The use of very sensitive and accurate molecular methods has
enabled the detection of large rhizobial diversity. Molecular biotyping and characterization the Bradyrhizobium, isolates from eleven varieties of soybean from agricultural field of Sehore district of Madhya Pradesh is done using 16S
rDNA typing. Bradyrhizobia were identified genetically by determining the %Guanine plus Cytosine content of the whole genome,
followed by 16S rDNA-RFLP analysis % Guanine plus Cytosine content of all the Bradyrhizobium isolates reflects similarity at generic level among all Bradyrhizobial isolates. Restriction Fragment Length Polymorphism
(RFLP) further showed a considerable level of genetic diversity among the Bradyrhizobial isolates. PCR-RFLP of 16S rDNA supported
existence of two divergent groups among indigenous Bradyrhizobial isolates, at similarity level of 66, and 75 and 74% of similarity
within the group. The technique used was helpful in characterizing Bradyrhizobium isolates to be used as inoculants for improving productivity of agricultural land of Madhya Pradesh (India). |
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Keywords: | % Guanine plus Cytosine content (% G + C) 16S rDNA Bradyrhizobium sp DNA fingerprinting Genetic diversity Soybean |
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