The use of a multiple antigen peptide to detect a minichromosome maintenance protein in pea (Pisum sativum) |
| |
Authors: | Michael S Munns Karen Moore Lyne Jossé Paul N Fitchett and John A Bryant |
| |
Institution: | (1) Peptide and Protein Research Consultants, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG, UK;(2) School of Biological Sciences, University of Exeter, Exeter, EX4 4QG, UK |
| |
Abstract: | A 13-residue oligopeptide corresponding to a conserved region of the MCM family of proteins was synthesised as a multiple antigen peptide in which eight copies of the peptide were conjugated to an oligo-lysine core. The multiple antigen peptide was used for raising antibodies. Western blots of the polypeptides present in the DNA polymerase–primase complex from pea (Pisum sativum L.) were challenged with the antibodies which, even at a dilution of 1:5000, clearly revealed a polypeptide of approximately 62 kDa. |
| |
Keywords: | DNA polymerase Multiple antigen peptide MCM protein Pea Pisum sativum |
本文献已被 SpringerLink 等数据库收录! |
|