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Radioimmunoassay of Gibberellins A5 and A20 |
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| Authors: | Yamaguchi Isomaro; Nakagawa Ryusuke; Kurogochi Shin; Murofushi Noboru; Takahashi Nobutaka; Weiler Elmar W |
| Institution: | 1Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan 2Nihon Tokushu Noyaku Co., Ltd Yuki-shi, Ibaragi-ken 307, Japan 3Pflanzenphysiologie, Universitaet Osnabrutck D-4500 Osnabrueck, Federal Republic of Germany |
| Abstract: | Polyclonal anti-GA5-antiseruin and anti-GA20-antiserum wereprepared by immunizing rabbits with conjugates of N-GA5-ß-alanylBSA and N-GA20-ß-alanyl BSA. By radioimmunoassay usingthese antisera, GA5 and GA20 in developing fruits of Pharbitisnil Chois were analyzed after several purification steps andthe results were compared with those obtained by GC-MS to examinethe reliability of the analysis by radioimmunoassay. The analyticalresults by radioimmunoassay did not agree with those by GC-MSuntil two-step purifications by HPLC, indicating that samplesmust be suitably purified prior to radioimmunoassay to obtainreliable results. (Received November 13, 1986; Accepted April 17, 1987) |
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