Mapping of some genes involved in C-1 metabolism in the facultative methylotroph Methylobacterium sp strain AM1 (Pseudomonas AM1) |
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| Authors: | P K Tatra P M Goodwin |
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| Institution: | (1) Department of Biological Sciences, North East Surrey College of Technology, Reigate Road, Ewell, KT17 3DS Epsom, Surrey, UK;(2) Present address: Department of Microbiology, London Hospital Medical College, Whitechapel, London, UK |
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| Abstract: | R68.45 mediated mobilisation of the chromosome of Methylobacterium sp strain AM1 has been investigated. High frequencies of cotransfer of four genes required for C-1 metabolism with the genes coding for streptomycin, phosphonomycin and cycloserine resistance were demonstrated. A preliminary map of this region has been constructed on the basis of the results of three and four factor crosses showing that not all the C-1 genes are contiguous.Abbreviations Str
streptomycin
- Pho
phosphonomycin
- Cyc
cycloserine
- Tc
tetracycline
- Km
kanamycin
- Cb
carbenicillin
- Ade
adenine
- Thi
thiamine
- Met
methionine |
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| Keywords: | Methylotroph Methylobacterium sp strain AM1 Pseudomonas sp strain AM1 Plasmid R68 45 |
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