The type antigen III of group F streptococci separation of group and type antigens and partial characterization of type III antigen |
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Authors: | J M N Willers M F Michel J H J Huis in ’t Veld G H J Alderkamp |
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Institution: | (1) Laboratory of Microbiology, State University of Utrecht, Catharijnesingel 59, Utrecht, The Netherlands;(2) Present address: Departmen tof Clinical Microbiology, Medical Faculty, Rotterdam, The Netherlands |
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Abstract: | Polysaccharides extracted from Z3III streptococci either with formamide or with dilute hydrochloric acid or isolated from the growth medium could be fractionated
in type III- and group Z3 antigens by alcohol precipitation. No good separation could be obtained from TCA extracts. When the same extractions and
fractionations were applied to streptococci carrying type III antigen, but different group antigens, good separations were
again obtained of all formamide extracts, but not of all hydrochloric acid extracts. The group antigens showed a rhamnose
content of at least 50% and contained hexosamines. Type III antigens contained mainly rhamnose, glucose and galactose in relative
amounts of approximately 1:2:3. Analysis of the methylated type III antigen suggests it to be a polysaccharide with a linear
structure. Type III antigen isolated from the medium was characterized not only by a different sugar ratio, but also by its
fucose content of 20%. In some cases the purified polysaccharides contained considerable amounts of glycogen-like material.
Partial acid hydrolysis of the type III antigen extracted with formamide yielded a great number of oligosaccharides. Analyses,
inhibition reactions and methylation studies gave indications that the most probable structure of a determinant group of type
III antigen is β-glucosyl-(1-6)-galactosyl-(1-6)-galactosyl-(1-3)-rhamnose. The possibility of the existence of a second determinant
group is discussed. |
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